Universität Salzburg (PLUS)
The Department of Molecular Biology at the University of Salzburg, a leading academic institution for research and teaching in biology in Austria, is an integral part of the priority program "BioSciences and Health" of the University, focusing on the topics allergy research, immunology, bioinformatics, genomics and structural biology.
Investigation of the molecular and cellular basis of different diseases serves as the common denominator. The priority program is strongly supported by the research emphases and infrastructure contributed by the recently established Division of Chemistry and Bioanalytics. The Division of Chemistry and Bioanalytics of the Department is equipped with state-of-the-art technology enabling the high-throughput analysis of proteomes and metabolomes. Main tasks in the project: PLUS mainly contributes to the generation of differential toxicity profiles following exposure of cell models to nanoparticles at the metabolome and proteome level for WP10. The analytical strategy of toxicity profiling comprises (1) cell quenching and metabolite extraction, protein isolation in the pellet and (2) identification and differential quantification of at least 800-1000 metabolites by direct-infusion high-resolution mass spectrometry and of 800-1000 proteins by high-performance liquid chromatography-Orbitrap mass spectrometry. The generated differential metabolite and protein patterns will be transferred to the data evaluation partners for processing and pathway modelling. The key result will be the identification of metabolic/proteomic pathways and selected biomarkers that reflect the effect of nanoparticles on cellular model systems.
Prof. Dr. Christian Huber
Fachbereich Molekulare Biologie
Hellbrunner Straße 34
Tel: +43 662 8044 5738
Fax: +43 662 8044 5751